FLIM X16 TCSPC Detector - LaVision BioTec GmbH
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FLIM X16 TCSPC Detector
LaVision BioTec‘s FLIM X16 TCSPC detector combines the advantages of intensified CCD cameras and PMT based FLIM detectors – it is fast and delivers confocal resolution even in deep tissue imaging.
The image acquisition time of a PMT based FLIM [Fluorescence Lifetime Imaging Microscopy] system is limited by its maximum photon counting rate that is typically between 1-
LaVision BioTec’s TCSPC system includes:
Liquid light guide for optical coupling to the microscope
16x PMT-
array Analog electronics including 16 CFDs [Constant Fraction Discriminator]
Two 8-
channel TDCs [Time To Digital Converter] 1 FPGA [Fast Programmable Gated Array] including USB 2 interface
ImSpector software package
Fast FLIM imaging at 76 Mhz
High sensitivity and spatial resolution
Straightforward integration via liquid light guide
Complete software package including phasor plot
Input Channels
To synchronize the FLIM X16 to the LSM it has to be triggered by the pulsed laser and the scanner.
The FLIM X16 provides 3 TTL inputs for:
1. Laser,
2. Frame and
3. Line Trigger.
Computer Interface and Software
• USB 2 interface to the computer
• LaVision BioTec‘s ImSpector Software package proceeds TCSPC data to get real time FLIM images including Phasor Plots
Electronics |
|
Total counting rate |
76 MHz |
# of channels |
16 |
Dead time/channel |
5.5 ns |
Max counting rate [burst] |
>2.5 GHz |
Multi- |
yes |
Temporal bin size |
80 ps |
PMT array detector |
|
Number of channels |
16 |
Arrangement |
rectangular (4x4) |
Spectral response |
300- |
Time Resolution [FWHM] |
300 ps |