FLIM X16 - 78 MHz TCSPC Detector


LaVision BioTec‘s FLIM X16 TCSPC detector combines the advantages of intensified CCD cameras and PMT based FLIM detectors – it is fast and delivers confocal resolution even in deep tissue imaging.

  •  Fast FLIM imaging at 76 MHz
  •  High sensitivity and spatial resolution
  •  Straightforward integration via liquid light guide
    into the TriM Scope II
  •  Complete software package including phasor plot and fluorescence lifetime analysis

LaVision BioTec’s TCSPC system 


  •   Liquid light guide for optical coupling to the microscope
  •   16x PMT-array
  •   Analog electronics including 16 CFDs (Constant Fraction Discriminator)
  •   Two 8-channel TDCs (Time To Digital Converter)
  •   1 FPGA (field programmable gate array logic)  including USB 2 interface
  •   ImSpector software package with analysis tools from mono exponential fits, multi exponential fits, and lifetime unmixing to phasor plot analysis

Idea behind FLIM X16


The image acquisition time of a PMT based FLIM (Fluorescence Lifetime Imaging Microscopy) system is limited by its maximum photon counting rate that is typically between 1-8 MHz. FLIM systems that are based on intensified CCD cameras overcome this restriction – but sensitivity and spatial resolution in 3D microscopy applications is limited. LaVision BioTec‘s PMT based FLIM X16 TCSPC (Time Correlated Single Photon Counting) detector combines both advantages – it is fast and sensitive by using an PMT array of 16 channels and two 8-channel TDCs. These advanced electronics and detectors provide 76 MHz photon counting rate (short term bursts up to 2.5 GHz). Therefore, the FLIM X16 TCSPC is the perfect choice for laser-scanning microscopes. 

Mcf7 Cells - left: intensity image, right: fluorescence lifetime image

Electronics
Total counting rate76 MHz
# of channels16
Dead time/channel5.5 ns
Max counting rate [burst]>2.5 GHz
Multi-hit abilityyes
Temporal bin size

80 ps 

PMT array detector 
 
Number of channels16
Arrangementrectangular (4x4)
Spectral response300-800 nm 
Time Resolution [FWHM]

300 ps (depends on detector choice)