Objective lenses

Refractive index adapted objective lenses

Imaging large samples needs certain procedures to reduce opacity. Some organisms like Zebra Fish larvae are mostly transparent by nature, but the majority of samples are opaque, making attempts to image a sample in depth difficult or impossible.

Considering ex vivo samples, there are two main principles of creating translucent samples that have been established to this day. In the case of organic solvent clearing, the principle of operation is equalizing the refractive index of sample and immersion solution. On the other hand, the sample may be cleared by using aqueous buffers which have a certain depolymerizing effect on structures like lipid chains.

However the different clearing protocols are based on solutions of certain refractive indices (RI). For this reason a cleared sample will finally also have a specific RI which is most likely not 1.33 as that of water. To still ensure best imaging quality the objective lens has to be optimized for the RI of the sample and the immersion liquid. LaVision BioTec took this into account and created different series of RI adaptable objective lenses meeting the needs of today’s clearing and imaging protocols.

Objective lenses optimized for long working distance

LWDO series

Objective lenses of the LWDO series are characterized by a long working distance and a refractive index matching range from 1.45 to 1.62.

Objective lens
MagnificationRI range
WD [mm]
NA Part #
LWDO 2x
2x1.45 - 1.57
120.14208044
LWDO 4x
4x1.45 - 157
100.28208045
LWDO 20x
20x1.45 - 1.57
5.50.45208046


LWDO 2x

LWDO 4x

LWDO 20x

Objective lenses for broad RI range

LVMI-Fluor series

An increased NA and a broad refractive index matching range including 1.33 are characteristics of the LVMI-Fluor series objective lenses.

Objective lens
MagnificationRI range
WD [mm]
NA Part #
LVMI-Fluor 1x
1x1.33 - 1.5790.1207513
LVMI-Fluor 4x
4x1.33 - 1.57
5.60.3205852
LVMI-Fluor 12x
12x1.33 - 1.57
8.5 - 10.5
0.532080XX



The LVMI-Fluor 12x objective lens can be equipped with different refractive index range dipping caps.

RI range
Immersion media
WD [mm]
Part #
1.33 - 1.41
water, aqueous buffers
8.5208046
1.42 - 1.48
CLARITY, CUBIC, Scale
9208049
1.49 - 1.57
BABB, DBE, DISCO, ECi
10.5208050


LVMI-Fluor 1x

LVMI-Fluor 4x

LVMI-Fluor 12x