iDISCO mouse embryo
Motoneurons & sensory neurons
Visualization of motoneurons and sensory neurons with tag-1 and ChAT immunolabelling.
Courtesy of Chloe Dominici and Alain Chédotal, INSTITUT DE LA VISION, Paris, France
iDISCO mouse brain
Short iDISCO+ demo
This video goes through a few types of samples processed with iDISCO+ whole-mount immunolabeling and clearing:
- Tyrosine Hydroxylase staining in an adult mouse brain
- ChAT-cre , Ai14 stained for Tomato, to reveal the cholinergic system in an intact adult mouse brain
- Hb9-GFP embryo stained for TrkA and GFP, to reveal motor and nociceptive fibers.
Courtesy of Nicolas Renier
3D data collection
Neurobiologists from the Vision Institute in Paris (P.I., Dr Alain Chédotal) and from JPArc in Lille (P.I., Dr Paolo Giacobini) collected 3D data of immunolabeled and transparent human embryos to understand birth defects, of structural or functional origin.