Objective Lenses

Ideal for Cleared Samples

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Background


Two- and three-dimensional deep tissue imaging usually requires clearing while keeping the sample’s structure intact. Most of today’s clearing protocols involve agents and immersion media with specific optical characteristics (high RIs up to 1.57  and specific dispersions), which have to match those of the objective lens for optimum results. Additionally, deep tissue imaging requires large free working distances. Conventional water, glycerin or oil immersion lenses do not meet these requirements.

Key Parameters


NA

Numerical Aperture Determines how much light enters the objective; the higher, the better the resolution
WD Working Distance Distance between front lens element and focal plane; the depth in which a researcher can image
RI Refractive Index Determines how light propagates through the immersion medium
Dispersion Change of RI with wavelength

Flat-Field-Correction


The flat-field-correction of LaVision BioTec’s MI PLAN objective lenses guarantees a flat focal plane matching the optical axis of a light sheet.

Apochromatic Multi-Immersion Planar Objective Lenses

MI PLAN Series

Highlights


  • Tailored to the optical characteristics (RI and dispersion) of a wide range of immersion media with RIs from 1.33 (water) to 1.57 (organic solvents)
  • Correction of chromatic aberration
  • Resistance against common media used for tissue clearing
  • Long WDs for deep tissue imaging of large samples
  • Correction of curvature of field (flat-field-correction) for images that are in common focus throughout the field of view
  • Highly suitable for light sheet microscopy

Three-dimensional image of a mouse cortex demonstrating the high isotropic resolution of LaVision BioTec’s MI PLAN objective lenses


Objective Lens Magnification NA Dipping Cap RI Range of
Imm. Medium
Clearing Method WD [mm]
1.1x NA 0.1 MI PLAN * 1.1x 0.1 DC40 WD16 AB 1.33 - 1.49 Aqueous buffers, e.g.:
CLARITY, CUBIC, ScaleS
16
DC57 WD17 O 1.50 - 1.57 Organic solvents, e.g.:
BABB, DBE, DISCO, Eci
17
LVMI-Fluor 4x/0.3 WD6 4x 0.3  - 1.33 - 1.57 Aqueous buffers,
Organic solvents
5.6
12x NA 0.53 MI PLAN * 12x 0.53 DC33 WD8.5 W 1.33 - 1.41 Aqueous buffers 8.5
DC49 WD10.9 AB 1.42 - 1.48 Aqueous buffers e.g.:
CLARITY, CUBIC, ScaleS
10.9
  DC57 WD10 O 1.49 - 1.57 Organic solvents, e.g.:
BABB, DBE, DISCO, Eci
10

Objective Lenses Optimized for Long Working Distances

LWDO series

Objective lenses of the LWDO series are characterized by a long working distance and a refractive index matching range from 1.45 to 1.62.

Objective lens
MagnificationRI range
WD [mm]
NA Part #
LWDO 2x
2x1.45 - 1.57
120.14208045
LWDO 4x
4x1.45 - 157
100.28208044
LWDO 20x
20x1.45 - 1.57
5.50.45208046


LWDO 2x

LWDO 4x

LWDO 20x