TriM Scope II Series
Components
Lasers
A large variety of lasers can be accommodated, either alone or in combination: classical TiSa (680-1080 nm), ultra-wide tunable (660-1320 nm), OPO (1010-1340 nm), and fixed-wavelength femtosecond (1040 or 1070 nm), visible lasers for phototreatment
This enables high flexibility regarding fluorophores and optogenetic actuators with different excitation ranges, as well as simultaneous excitation of several dyes.
Optional dispersion compensation keeps laser pulse length short, which guarantees efficient excitation with any type of optical layout and objective.
A common problem when using different laser wavelengths for inspection of numerous dyes or for simultaneous imaging and photomanipulation is a mismatch of focal planes. The TriMSope II can be equipped with adjustable telescope optics to solve this problem.
| Type | Wavelength [nm] | Models (examples) | Application | Probes (examples) |
| Titanium-Sapphire | 690-1080 | Coherent Chameleon Ultra II, Spectra Physics MaiTai | Fluorescence | GFP, GCaMP, YFP |
| Optogenetics & Uncaging | ChR2, MNI-Glu, RuBi-GABA | |||
| SHG | intrinsic | |||
| OPO | 1010-1340 | Coherent Chameleon MPX (pumped with Ultra II) | Fluorescence | RFP, mCherry |
| Optogenetics | C1V1 | |||
| SHG, THG | intrinsic | |||
| Ultra-wide tunable | 680-1300 | Spectra Physics InSight X3, Coherent Chameleon Discovery | Fluorescence | GFP, GCaMP, YFP, RFP, mCherry |
| Optogenetics & Uncaging | ChR2, MNI-Glu, RuBi-GABA, C1V1 | |||
| SHG, THg | intrinsic | |||
| Fixed-Wavelength Femtosecond | 1045, 1070 | Coherent Fidelity-2, Spectra Physics HighQ-2 | Optogenetics | C1V1, ReaChR |
| Visible lasers (1-photon) | 488, 532, 561, 594, 637, etc. | Coherent Obis | Fluorescence | GFP, YFP, mCherry |
| Optogenetics & Uncaging | ChR2, MNI-Glu, Halorhodopsin, Chrimson |