TriM Scope II scanner


The TriM Scope II offers different combination of galvo and resonant scanners:       

  •   2 galvanometric scanners with 2.5 frames/sec @ 512x512 px resolution in the unidirectional mode 
  •   Fast resonant scanner with > 30 frames/sec @ 512x512 px resolution       
  •    The alternative use of galvo or resonant scanner       
  •   A second independent galvanometric scanner additional to the existing galvo or resonant scanner

Beside the standard configuration with two galvo scanners for 3D imaging, line scanning and local photo-treatment, the user can add a resonant scanner to its system. With more than 30 frames/sec @ 512x512 pixel resolution, the resonant scanner allows fast imaging of e.g. calcium signals.

By using the resonant scanner in combination with a piezo driven z-stepper, fast 3D imaging of large volumes is possible. This enables e.g. the observation of signal forwarding in the 3D neuronal networks of living animals or cell movement trough the tissue.

For photo-treatment with a galvo scanner and fast imaging with a resonant scanner, a second independent galvo scanner can be added to the TriM Scope II.

This configuration enables fast imaging with the resonant scanner during photo-activation, stimulation, or optogenetic modulation via the independent galvo scanner. For photo-treatment with a galvo scanner and fast imaging with a resonant scanner, a second independent galvo scanner can be added to the TriM Scope II. This configuration enables fast imaging with the resonant scanner during photo-activation, stimulation, or optogenetic modulation via the independent galvo scanner.

Scanfeatures


  • ROI-scanning (arbitrary speed and field dimension)
  •  Off center fields, large scans, line averaging
  •  Line scanning (high speed single line, polygon scanning)
  •  Multipoint access, mixed scan modes
  •  Photo-treatment of single and multiple ROIs, laser cutting
  •  Fast resonant scanning
  •  Fast 3D imaging with resonant scanner and piezo driven z-stepper
  •  Independent 2nd galvo scanner for simultaneous imaging and treatment

Scan rates of the 2 axis galvanometric XY-scanner


Max. number of pixel: 14500 x 14500 unidirectional,
1000 x 1000 bidirectional

Scan rates @ 250 x 250 pixel resolution in the unidirectional scan mode

FOV 
[µm x µm], 20x Objective lens
max scanner frequency [Hz]min frame time [ms]
max frame rate [Hz] 
500 x 500
800397 2.5
400 x 40012002943.4
300 x 30016002414.1
200 x 20020002074.8
100 x 10024001905.2
50 x 50
26001785.6

Scan rates @ 250 x 250 pixel resolution in the bidirectional scan mode

FOV 
[µm x µm], 20x Objective lens
max scanner frequency [Hz]min frame time [ms]
max frame rate [Hz] 
500 x 500
20001297.7
400 x 40025001069.3
300 x 30030008911.1
200 x 20035007812.8
100 x 10040006615.0
50 x 50
40006615.0