TriM Scope II Series

2-Photon Microscopy Systems of Modular Design for Deep Penetration into Living Tissue

Two-photon microscopy provides three-dimensional optical sectioning with unmatched penetration depth, but minimal photobleaching and -toxicity. Thus, two-photon microscopy allows investigations of samples like living embryos, whole organs, and even entire animals in vivo that would not be possible with any other imaging technique.

LaVision BioTec’s TriM Scope II series is a modular platform for 2-photon imaging and optogenetic manipulation. Being highly customizable, it can be used for a large variety of applications, ranging from developmental biology, cancer research and immunology to neuroscience. Many different types of measurements are possible:

  • Multi-color imaging
  • 3D time lapse over hours
  • Fast calcium monitoring
  • and many more...

The TriM Scope comes with “ImSpector”, a flexible software package for hardware integration and fast data acquisition.

Any TriM Scope can be deliverd tailored to specific demands from the beginning, or it can be upgraded later on by adding lasers, scanhead modules, detectors, and special components for in vivo imaging, or by integration of 3rd party components.

TriM Scope II Series

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TriM Scope II Series

Configurations

Upgradable Basic System

Sample Rotation by 360 °

Combination of Behavioral Tests with In Vivo Imaging

Looking at Samples from Below

TriM Scope II Series

Components

TriM Scope II Series

Multi Photon Technology

The basic principle of two-photon absorption keeps excitation confined to the focal plane. Moreover, instead of visible excitation light infrared wavelengths can be used, which have less scattering and lower phototoxicity in biological tissue. These characteristics lead to brilliant spatial resolution, whereas photobleaching and -damage are kept at a minimum. Imaging can be done in deep layers, much beyond those reached with confocal microscopy, in particular in highly scattering tissue. Therefore, two-photon microscopy presents an excellent method for imaging in thick slices of tissue or entire organs, and particularly in intact animals.

Two-photon excitation depends on the simultaneous absorption of two photons, so the resulting fluorescence emission intensity depends on the square of the excitation intensity keeping signals confined to the focal plane