TriM Scope II "Inverted & Doubleheader"

Looking at Samples from Below

Inverted microscopes are constructed with the objective underneath the stage, pointing upward, so as to view the sample from below. In some applications, inverted stands are superior to upright stands:

  • Imaging of cultured cells, which adhere to the bottom of a dish, spread across one focal plane, while having access to large amounts of medium
  • Applications where access to the sample from the top is needed (Liquid exchange, micropipettes)
  • Sterile applications as there is no contact between the objective and the sample

With its TriM Scope II "Inverted", LaVision BioTec offers a stand-alone inverted 2-photon microscope.

The Doubleheader is a system in which an upright and an inverted microscope share a common scanhead and excitation laser. This unique feature makes it a perfect, cost-effective instrument for multi-user environments, or whenever a versatile 2-photon platform for various applications is required.

LaVision BioTec’s “Inverted” and “Doubleheader” provide high flexibility and numerous upgrade options: Resonant & Galvo Scanners, up to 6 detection channels for each stand (plus 2 at transmission port for upright), FLIM module, etc.

TriM Scope II "Inverted"

Stand-Alone Inverted 2-Photon Microscope

The TriM Scope II can be equipped with one of the following  inverted stands:

  • Nikon Ti-U
  • Olympus BX 51 WI
  • Zeiss Axio Observer.


TriM Scope II "Doubleheader"

Combined Inverted and Upright 2-Photon Microscope Sharing One Scan Head and the Same Excitation Laser(s)

The TriM Scope II "Doublheader" includes an upright and an inverted stand 

  • sharing one scan head
  • sharing the excitation laser(s).

Switching between the inverted and upright mode can be easily done within seconds without further alignment.


Z-stack of a bone marrow chimera animal that highlights the lymphatics and vasculature within a lymph node.
[courtesy of Ann Haberman and David Gonzalez, School of Medicine, Yale University, USA]